Comparing antigenic similarity for protein antigens
CE-BLAST is presented here as an unsupervised program to compare the antigenic similarity between any conformational epitopes in protein antigens, especially for newly emerging pathogens. Large scale validation indicated that CE-BLAST has the potential for future infectious diseases surveillance, vaccine development, pathogen serological clustering and cross-reactive epitope discovery. Users could upload the query structure and make antigenic comparison through three built-in epitope libraries or self-defined library. Currently, the three built-in epitope databases including: 1) simulated hemagglutinin (JA) epitope for influenza virus (A/H1N1 & H3N2), 2) envelope (E) protein database for dengue virus (DENV) and zika virus (ZIKV), and 3) known conformational epitope database derived from the Protein Data Bank (PDB). Users can also define their own epitope sites for HA protein and E protein to start CE-BLAST. Furthermore, multiple epitopes can be uploaded to search against each other by users.
*The workflow covers several steps. The next step will only pop out when the previous step is finished. Please try to preprocess your files if error messages received.
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